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Intraductal Injection til Lokaliseret Drug Delivery til Mouse mælkekirtlerne
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Biology
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JoVE Journal Biology
Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

Intraductal Injection til Lokaliseret Drug Delivery til Mouse mælkekirtlerne

DOI:
10.3791/50692-v

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06:39 min

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October 04, 2013

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, ,
1Vascular Biology Program, Department of Surgery,Boston Children’s Hospital and Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering,Harvard University, 3Harvard School of Engineering and Applied Sciences

Chapters

  • 00:05Title
  • 01:16Pre-operative Preparation
  • 02:48Intraductal Injection and Analysis of Mammary Gland Tissue
  • 05:02Results: Mammary Gland Injections Enable Localized Delivery of Molecules
  • 06:15Conclusion

Summary

Automatic Translation

Automatic Translation

En protokol for non-invasiv intraductal levering af vandige reagenser til muse brystkirtel beskrevet. Metoden udnytter lokaliseret injektion i brystvorterne af mælkekirtlerne rettet brystkirtler kanaler specifikt. Denne teknik kan tilpasses til en række forskellige forbindelser, herunder siRNA, kemoterapeutiske midler og små molekyler.

Tags

Intraductal InjectionLocalized Drug DeliveryMouse Mammary GlandEvans BlueFluorescent ReagentsNon-invasiveSiRNAChemotherapeutic AgentsSmall Molecules

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