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Gjær Luminometric og
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Gjær Luminometric og

Article DOI: 10.3791/50816-v • 12:09 min • December 31st, 2013
December 31st, 2013 •
1, 1, 1, 1,2
1Laboratory of Cell and Molecular Biology, University of Wisconsin – Madison, 2Department of Genetics, University of Wisconsin – Madison

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For å komplettere brukte metodene for å studere TRPV4 tallet, er to metoder beskrevet: Its mechanosensitivity kan studeres av Ca 2 +-aequorin luminometry i transgen gjær ved hypo-osmotisk utfordring. Det kan også undersøkes i TRPV4-RNA injisert Xenopus oocytter ved hel-celle-to-elektrodespenningsklemme eller lapp klemmes fast i on-celle eller skåret ut-modus.

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Grunnleggende Protocol Eukaryota Archaea Bakterier miljø-og biovitenskap (General) Mechanosensation Ionekanaler lipider patch clamp, gjær luminometry kraft sensing spenning klemme TRPV4 elektrofysiologi
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