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Maya lüminometrik ve
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Maya lüminometrik ve

Article DOI: 10.3791/50816-v • 12:09 min • December 31st, 2013
December 31st, 2013 •
1, 1, 1, 1,2
1Laboratory of Cell and Molecular Biology, University of Wisconsin – Madison, 2Department of Genetics, University of Wisconsin – Madison

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TRPV4 en incelemek için yaygın olarak kullanılan yöntemler tamamlamak için, iki yöntem tarif edilmiştir: Onun mechanosensitivity hipo-ozmotik tehdidi üzerine transgenik maya Ca 2 +-akorinden luminometri ele alınabilir. Ayrıca TRPV4-RNA incelenebilir on-hücre ya da kesilmiş modunda kelepçe bütün hücre iki elektrotlu voltaj kelepçe veya yama ile Xenopus oosit enjekte edilir.

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Temel Protokol Sayı 82 Eukaryota Arkea Bakteri Yaşam Bilimleri (Genel) Mechanosensation İyon kanalları Yağlar yama kelepçe, maya luminometri kuvvet algılama gerilim kelepçe TRPV4 elektrofizyoloji
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