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单线粒体超氧闪烁在完整心脏或共聚焦成像<em>在体内</em
JoVE Journal
Biology
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JoVE Journal Biology
Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo
DOI:

12:06 min

November 05, 2013

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Chapters

  • 00:05Title
  • 01:49Confocal Imaging of Skeletal Muscles In Vivo
  • 04:30Confocal Imaging of the Perfused Mouse Heart
  • 07:18Image Processing and Data Analysis
  • 09:20Representative Mitochondrial Superoxide Flash Imaging Results
  • 11:23Conclusion

Summary

Automatic Translation

共聚焦扫描显微镜是适用于在成像灌注心脏或骨骼肌线粒体单事件活的动物。单个线粒体过程,如超氧化物闪烁和膜电位的波动进行实时监控,使线粒体功能的评价在生理相关的背景和病理过程中的扰动。

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