Journal
/
Immunology and Infection
/
In vitro Ensaio de cocultura para avaliar migração trans epitelial de neutrófilo induzida por patógenos
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration
Please note that all translations are automatically generated. Click here for the English version.

In vitro Ensaio de cocultura para avaliar migração trans epitelial de neutrófilo induzida por patógenos

português

Automatically Generated

17,302 Views

•

14:14 min

•

January 06, 2014

DOI:

10.3791/50823-v

DOI:
10.3791/50823-v

•

14:14 min
January 06, 2014

•

8 Views
, , ,
1Department of Pediatrics,Harvard Medical School, 2Department of Pediatric Gastroenterology,MGH for Children, 3Mucosal Immunology and Biology Research Center,Massachusetts General Hospital

Summary

Automatically generated

A migração trans-epitelial de neutrófilos em resposta à infecção bacteriana mucosa contribui para lesão epitelial e doença clínica. Foi desenvolvido um modelo in vitro que combina patógenos, neutrófilos humanos e camadas de células epiteliais humanas polarizadas cultivadas em filtros transwell para facilitar investigações para desvendar os mecanismos moleculares que orquestram esse fenômeno.

Explore More Videos

In Vitro CocultureNeutrophil Trans-epithelial MigrationMucosal ImmunityPathogenic InfectionPolarized Epithelial MonolayerNeutrophil InfiltrationInflammatory ResponseTherapeutic Targets

Read Article

Cite this Article

Kusek, M. E., Pazos, M. A., Pirzai, W., Hurley, B. P. In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration. J. Vis. Exp. (83), e50823, doi:10.3791/50823 (2014).

Download .ris file

Copy

Share Video

.

Copy