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Bioengineering
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芯片上的压力诱导抗生素易感性测试
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Stress-induced Antibiotic Susceptibility Testing on a Chip
Please note that all translations are automatically generated.
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芯片上的压力诱导抗生素易感性测试
DOI:
10.3791/50828-v
•
12:41 min
•
January 08, 2014
•
Maxim Kalashnikov
,
Jennifer Campbell
,
Jean C. Lee
,
Andre Sharon
3
,
Alexis F. Sauer-Budge
4
1
Fraunhofer USA Center for Manufacturing Innovation
,
2
Division of Infectious Diseases, Department of Medicine, Brigham and Women’s Hospital
,
Harvard Medical School
,
3
Department of Mechanical Engineering
,
Boston University
,
4
Department of Biomedical Engineering
,
Boston University
Chapters
00:05
Title
01:40
Making the Poly(dimethylsiloxane) (PDMS) Layer
02:29
Assembling the Flow Cell
03:41
Loading the Bacterial Suspension
05:08
Loading the Solutions
06:15
Image Acquisition
08:02
Disinfecting the Flow Cell and Counting the Bacteria
09:21
Results: Representative Bacterial Susceptibility Imaging and Survival Data
12:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们开发了一个用于快速抗生素易感性检测的微流体平台。流体以高速通过在微流体通道底部固定的细菌。在压力和抗生素的存在下,易感菌株会迅速死亡。然而,耐药细菌可以在这些紧张的条件下存活下来。
Tags
Microfluidics
Antibiotic Susceptibility Testing
Stress-based Testing
Bacterial Culture
PDMS
Cell Viability
Fluorescence
Image Processing
Mechanical Stress
Enzymes
Microarray Substrates
Drug Development
Bacterial Suspension Experiments
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