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Bioengineering
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隔离细胞脂滴:两个纯化技术从酵母细胞与人类的胎盘开始
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
Please note that all translations are automatically generated.
Click here for the English version.
隔离细胞脂滴:两个纯化技术从酵母细胞与人类的胎盘开始
DOI:
10.3791/50981-v
•
09:41 min
•
April 01, 2014
•
Jaana Mannik*
1
,
Alex Meyers*
2
,
Paul Dalhaimer
2
1
Department of Biochemistry and Cellular and Molecular Biology
,
University of Tennessee
,
2
Department of Chemical and Biomolecular Engineering
,
University of Tennessee
Chapters
00:05
Title
01:01
Preparing Placenta for Dissection
04:49
Homogenizing Placental Villous Cells and Isolating Lipid Droplets by Ultracentrifugation
07:43
Results: Isolation of Lipid Droplets from Placental Villous Cells
09:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
两种技术从1分离细胞的脂滴),酵母细胞和2)人类胎盘介绍。这两个程序的核心是密度梯度离心法,在这里可以用肉眼很容易地可视化含有液滴产生的浮层,提取,并通过Western印迹分析纯度量化。
Tags
Lipid Droplets
Density Gradient Centrifugation
Yeast Cells
Human Placental Villous Cells
Protein Isolation
Proteomic Analysis
Lipidomic Analysis
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