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隔离细胞脂滴:两个纯化技术从酵母细胞与人类的胎盘开始
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
DOI:

09:41 min

April 01, 2014

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Chapters

  • 00:05Title
  • 01:01Preparing Placenta for Dissection
  • 04:49Homogenizing Placental Villous Cells and Isolating Lipid Droplets by Ultracentrifugation
  • 07:43Results: Isolation of Lipid Droplets from Placental Villous Cells
  • 09:16Conclusion

Summary

Automatic Translation

两种技术从1分离细胞的脂滴),酵母细胞和2)人类胎盘介绍。这两个程序的核心是密度梯度离心法,在这里可以用肉眼很容易地可视化含有液滴产生的浮层,提取,并通过Western印迹分析纯度量化。

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