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隔离细胞脂滴：两个纯化技术从酵母细胞与人类的胎盘开始
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Bioengineering
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JoVE Journal Bioengineering
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
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隔离细胞脂滴：两个纯化技术从酵母细胞与人类的胎盘开始

DOI:
10.3791/50981-v

•

09:41 min

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April 01, 2014

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, ,
1Department of Biochemistry and Cellular and Molecular Biology,University of Tennessee, 2Department of Chemical and Biomolecular Engineering,University of Tennessee

Chapters

  • 00:05Title
  • 01:01Preparing Placenta for Dissection
  • 04:49Homogenizing Placental Villous Cells and Isolating Lipid Droplets by Ultracentrifugation
  • 07:43Results: Isolation of Lipid Droplets from Placental Villous Cells
  • 09:16Conclusion

Summary

Automatic Translation

Automatic Translation

两种技术从1分离细胞的脂滴），酵母细胞和2）人类胎盘介绍。这两个程序的核心是密度梯度离心法，在这里可以用肉眼很容易地可视化含有液滴产生的浮层，提取，并通过Western印迹分析纯度量化。

Tags

Lipid DropletsDensity Gradient CentrifugationYeast CellsHuman Placental Villous CellsProtein IsolationProteomic AnalysisLipidomic Analysis

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