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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Cecal legatura e la puntura indotta da sepsi come modello per studiare autofagia nei topi
 
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Cecal legatura e la puntura indotta da sepsi come modello per studiare autofagia nei topi

Article doi: 10.3791/51066
February 9th, 2014 •
1,3, 1, 2, 2, 1, 1
1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 2Department of Medicine, Renal Division, Brigham and Women's Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services, University of Athens Medical School, Evangelismos Hospital, Athens, Greece

Summary
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February 9th, 2014

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Sepsi sperimentale può essere indotta in topi usando la legatura del cieco e puntura metodo (CLP). Attuali protocolli per valutare autofagia in vivo nel contesto della sepsi CLP-indotta sono presentati qui: Un protocollo per misurare autofagia utilizzando topi (GFP)-LC3, e un protocollo per misurare formazione dell'autofagosoma mediante microscopia elettronica.

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