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盲肠结扎穿孔致脓毒症作为模型小鼠中自噬研究
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice
DOI:

06:40 min

February 09, 2014

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Chapters

  • 00:05Title
  • 01:45Cecal Ligation and Puncture
  • 03:44Tissue harvest and Fixation
  • 05:06Results: Autophagy in Mice after Cecal Ligation and Puncture
  • 06:05Conclusion

Summary

Automatic Translation

实验性败血症可以在小鼠体内利用盲肠结扎穿孔(CLP)方法来诱导。当前的协议,以评估在CLP诱导的脓毒症的上下文中的自噬体内这里提出:一个协议,用于使用(GFP)-LC3的小鼠测定自噬和协议,用于测量自噬体形成通过电子显微镜。

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