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Neuroscience
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检测持久性病毒的DNA在神经组织中的荧光的基因组与成绩单
原位
杂交结合免疫组化染色
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent
In situ
Hybridization Combined with Immunostaining
Please note that all translations are automatically generated.
Click here for the English version.
检测持久性病毒的DNA在神经组织中的荧光的基因组与成绩单
原位
杂交结合免疫组化染色
DOI:
10.3791/51091-v
•
13:22 min
•
January 23, 2014
•
Frédéric Catez
2,5
,
Antoine Rousseau
,
Marc Labetoulle
,
Patrick Lomonte
2,3
1
Virus and Centromere Team, Centre de Génétique et Physiologie Moléculaire et Cellulaire
,
CNRS UMR 5534
,
2
Université de Lyon 1
,
3
Laboratoire d’excellence
,
LabEX DEVweCAN
,
4
Institut de Virologie Moléculaire et Structurale
,
CNRS UPR 3296
,
5
Centre de Recherche en Cancérologie de Lyon, INSERM U1052
,
CNRS UMR 5286
Chapters
00:05
Title
02:27
Animal Infection
03:20
Trigeminal Ganglia Harvesting and Sectioning
05:02
DNA-FISH
10:08
Protocol Development and Applications
12:32
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们
原位
杂交协议建立一个荧光供于动物模型的组织切片中的持久DNA病毒基因组的检测。这个协议允许研究感染过程由codetection病毒基因组,它的RNA产物,以及单细胞内的病毒或细胞蛋白。
Tags
DNA-FISH
RNA-FISH
Immunofluorescence
HSV-1
Latency
Gene Expression Regulation
Single Cell Analysis
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