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分段微管的制备研究运动驱动的微管拆解完
JoVE Journal
Biology
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JoVE Journal Biology
Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
DOI:

12:20 min

March 15, 2014

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Chapters

  • 00:05Title
  • 01:21Manufacturing Reusable Flow Chambers
  • 02:55Preparing the Coverslips
  • 05:04Attaching Microtubule Seeds to the Coverslips
  • 08:04Growing Segmented Microtubules
  • 09:04Interpreting the Results
  • 11:59Conclusion

Summary

Automatic Translation

微管本质上是不稳定的聚合物,以及生长和缩短之间的切换是随机的并且难以控制。在这里,我们将介绍使用分段微管与photoablatable稳定上限协议。分割的微管的解聚可以以高的时间和空间分辨率被触发,从而协助运动与拆卸的微管末端的分析。

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