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JoVE Journal Medicine
Cerebrospinalvæske microRNA ved brug af kvantitativ real time PCR
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Cerebrospinalvæske microRNA ved brug af kvantitativ real time PCR

Article DOI: 10.3791/51172
January 22nd, 2014 •
1, 2, 1, 1
1Medical School and Stanley S. Scott Cancer Center, LSU Health Sciences Center, 2Department of Biomedical, Surgery and Dental Sciences, University of Milan

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January 22nd, 2014

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Vi beskriver en protokol af real time PCR til at profilere microRNA i cerebrospinalvæsken (CSF). Med undtagelse af RNA ekstraktionsprotokoller kan fremgangsmåden udvides til RNA ekstraheret fra andre legemsvæsker, dyrkede celler eller vævsprøver.

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