A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery,University of Pittsburgh, 2Department of Orthopedic Surgery,University of Pittsburgh, 3Nazarbayev University Research and Innovation System,Nazarbayev University, 4Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center,University of California at Los Angeles, 5Department of Cell Biology,Erasmus MC Stem Cell Institute, 6OHSU Center for Regenerative Medicine,Oregon Health & Science University, 7Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine,Queen’s Medical Research Institute and University of Edinburgh, 8David Geffen School of Medicine and the Orthopaedic Hospital Research Center,University of California at Los Angeles, 9Stem Cell Research Center, Department of Orthopedic Surgery and McGowan Institute for Regenerative Medicine,University of Pittsburgh
Chapters
- 00:05Title
- 01:53Muscle Biopsy Processing
- 02:42Muscle Dissociation and Cell Isolation
- 05:12Cell Labelling and Sorting
- 06:52Post-sorting Cell Culture
- 08:08Results: Human Blood-vessel-derived Stem Cell Subsets
- 10:00Conclusion
Blood vessels within human skeletal muscle harbor several multi-lineage precursor populations that are ideal for regenerative applications. This isolation method allows simultaneous purification of three multipotent precursor cell populations respectively from three structural layers of blood vessels: myogenic endothelial cells from intima, pericytes from media, and adventitial cells from adventitia.
