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二硝基苯磺酸/ TNBS结肠炎模型：提供洞察炎症性肠病和膳食脂肪的影响
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JoVE Journal Medicine
DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
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二硝基苯磺酸/ TNBS结肠炎模型：提供洞察炎症性肠病和膳食脂肪的影响

DOI:
10.3791/51297-v

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09:04 min

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February 27, 2014

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, , , , , ,
1Division of Gastroenterology,BC Children’s Hospital

Chapters

  • 00:05Title
  • 01:24Intra Rectal Dinitrobenzene Sulfonic Acid (DNBS) Administration
  • 02:52Collecting Tissues and Assessing Histological Damage in DNBS Challenged Mice
  • 04:27Myeloperoxidase (MPO) Assay of DNBS Challenged Intestinal Tissue
  • 05:25Results: Representative Effects of Dietary Oils on DNBS Colitis Severity of DNBS Challenged Intestinal Tissue
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

二硝基苯磺酸/ TNBS诱导的结肠炎提供了一种替代，并研究炎性肠病的病理生物学廉价的方法。在本文所讨论的协议概述DNBS的成功应用诱导结肠炎小鼠和大鼠，并允许一个深入研究宿主介导的肠道反应。

Tags

DNBSTNBSColitisInflammatory Bowel DiseaseDietary FatN-3 Fatty AcidsN-6 Fatty AcidsSafflower OilCanola OilFish OilWeight LossMucosal InflammationMyeloperoxidase

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