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核苷三磷酸 - 从合成到生化特征
JoVE Journal
Chemistry
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JoVE Journal
Chemistry
Nucleoside Triphosphates – From Synthesis to Biochemical Characterization
Please note that all translations are automatically generated.
Click here for the English version.
核苷三磷酸 - 从合成到生化特征
DOI:
10.3791/51385-v
•
15:22 min
•
April 03, 2014
•
Marcel Hollenstein
,
Christine Catherine Smith
,
Michael Räz
1
Department of Chemistry and Biochemistry
,
University of Bern
Chapters
00:05
Title
02:30
Synthesis of the Modified Nucleoside Triphosphates
05:56
HPLC Purification of the Modified Nucleoside Triphosphates
07:40
Primer Extension Reactions and TdT Polymerizations
11:05
Results: Biochemical Characterization of Nucleoside Triphosphates
14:02
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文所描述的协议旨在解释和删节的复杂路线导致修饰的三磷酸核苷的方法的许多障碍。因此,该协议有利于激活这些构建模块两者的合成及其可用性的实际应用。
Tags
Nucleoside Triphosphates
Phosphoramidite Precursors
Solid-phase Synthesis
Modified Nucleic Acids
Functional Tagging
Ribozymes
DNAzymes
Phosphorous(III)-based Reagents
Primer Extension Reactions
TdT Tailing Polymerization
Chemical Biology
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