JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
谱系重编程的成人大脑的周细胞源性细胞诱导成神经细胞
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
Please note that all translations are automatically generated.
Click here for the English version.
谱系重编程的成人大脑的周细胞源性细胞诱导成神经细胞
DOI:
10.3791/51433-v
•
09:36 min
•
May 12, 2014
•
Marisa Karow
,
Christian Schichor
,
Ruth Beckervordersandforth
,
Benedikt Berninger
4
1
Department of Physiological Genomics, Institute of Physiology
,
Ludwig Maximilians University Munich
,
2
Tumor Biology Lab, Neurosurgical Clinic
,
Ludwig-Maximilians University Munich
,
3
Institut für Biochemie, Emil-Fischer-Zentrum
,
Friedrich-Alexander-Universität Erlangen-Nürnberg
,
4
Institute of Physiological Chemistry and Focus Program Translational Neuroscience
,
Johannes Gutenberg University Mainz
Chapters
00:05
Title
01:34
Isolation and Culturing of Adult Human Brain Cells
02:55
In vitro
Expansion and Characterization of Adult Human Brain Cells
05:15
Retroviral Transduction of Pericyte-derived Cells and Reprogramming into Induced Neurons
06:41
Results: Reprogramming Adult Brain Cells into Induced Neurons
09:11
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
针对脑固有细胞直接谱系重编程提供了脑修复的新观点。在这里,我们描述了如何准备丰富的文化从成人大脑皮质脑居民周细胞,并通过转录逆转录病毒介导因子的表达Sox2的和ASCL1将这些转换引起的神经元的协议。
Tags
Lineage-reprogramming
Pericyte
Adult Human Brain
Induced Neurons
Direct Conversion
Neurogenesis
In Vitro Modeling
Brain Repair
Cerebral Cortex
PDGF Receptor-β
CD146
Sox2
Ascl1
Pericyte-derived Induced Neurons
Patch-clamp Recording
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
成人出生的神经元的整合研究
从人类多能干细胞的规范在前脑谷氨酸能神经元
中脑多巴胺神经元的数目在成年小鼠环境的调变
成人人成纤维细胞的推导和他们直接转换成可扩展的神经祖细胞
人类成人皮肤成纤维细胞诱导神经元高产培养的简单生成
利用 PiggyBac 载体将人诱导的多能干细胞 (Ipsc) 转化为功能性脊柱和颅内运动神经元
来自成人大脑的短期自由浮动切片培养
人类干细胞衍生内皮细胞和GABAergic神经元的迁移、化学吸引和共培养测定
从成人人类脑组织获取人类微胶质
人类诱导多能干细胞脑微血管内皮细胞的衍生、扩张、冷冻保存和特征化
Read Article