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Het gebruik van gaschromatografie tot veranderingen in de samenstelling van vetzuren Analyseer in Rat leverweefsel tijdens de zwangerschap
JoVE Journal
Chemistry
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JoVE Journal Chemistry
The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
DOI:

11:08 min

March 13, 2014

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Chapters

  • 00:05Title
  • 01:28Preparation of a Total Lipid Extract
  • 03:00Separation of Lipid Classes by Solid Phase Extraction
  • 05:19Preparation of Fatty Acid Methyl Esters from Cholesteryl Esters
  • 06:26Removal of Cholesterol Contamination and Gas Chromatographic Analysis
  • 08:02Results: Identification and Quantification of Fatty Acids in Rat Liver Tissue by Gas Chromatography
  • 10:24Conclusion

Summary

Automatic Translation

Zwangerschap leidt tot belangrijke veranderingen in de vetzuursamenstelling van maternale weefsels. Lipidenprofiel kan worden verkregen via gaschromatografie van de identificatie en kwantificatie van vetzuren in individuele lipideklassen onder ratten verschillende hoge en lage vetgehalte diëten tijdens de zwangerschap.

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