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14:43 min
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August 27, 2014
DOI:
1LENS – European Laboratory for Non-linear Spectroscopy,University of Florence, 2Chemistry Research Laboratory,University of Oxford, 3Department of Biology,University of Florence, 4Department of Physics and Astronomy,University of Florence, 5National Institute of Optics-National Research Council, Italy, 6International Center of Computational Neurophotonics
Summary
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Here we describe the instrumentation and methods for detecting single fluorescently-labeled protein molecules interacting with a single DNA molecule suspended between two optically trapped microspheres.
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Monico, C., Belcastro, G., Vanzi, F., Pavone, F. S., Capitanio, M. Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions. J. Vis. Exp. (90), e51446, doi:10.3791/51446 (2014).
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