JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
タンパク質-DNA相互作用の研究のための単一分子マニピュレーションとイメージングを組み合わせる
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
Please note that all translations are automatically generated.
Click here for the English version.
タンパク質-DNA相互作用の研究のための単一分子マニピュレーションとイメージングを組み合わせる
DOI:
10.3791/51446-v
•
14:43 min
•
August 27, 2014
•
Carina Monico
2
,
Gionata Belcastro
,
Francesco Vanzi
3
,
Francesco S. Pavone
4,5,6
,
Marco Capitanio
4
1
LENS – European Laboratory for Non-linear Spectroscopy
,
University of Florence
,
2
Chemistry Research Laboratory
,
University of Oxford
,
3
Department of Biology
,
University of Florence
,
4
Department of Physics and Astronomy
,
University of Florence
,
5
National Institute of Optics-National Research Council, Italy
,
6
International Center of Computational Neurophotonics
Chapters
00:05
Title
02:39
Optical Tweezers Setup with Nanometer Stability
04:40
Microfluidics
08:36
Combined Optical Trapping and Single-molecule Fluorescence Imaging Experiments
11:50
Results: Detection of Single Protein Molecules Interacting with a Single DNA Molecule
12:56
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
ここでは、二枚の光学トラップされたマイクロスフェアの間に懸架単一のDNA分子と相互作用する単一の蛍光標識タンパク質分子を検出するための機器および方法が記載されている。
Tags
Protein-DNA Interaction
Optical Tweezers
Single-molecule Fluorescence
Lactose Repressor
DNA Target Sequence
1D Diffusion
Molecular Motors
Single-molecule Manipulation
Single-molecule Imaging
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
トカゲの運動の速度と方向の視覚感度のテスト
コンピュータによって生成された動物モデルの刺激
二層弾性特性の変化をモニタリングするための単一分子法
共焦点イメージングのための成人心筋細胞の単離と遺伝子操作
核輸送の1分子イメージング
遺伝子調節の1分子蛍光イメージング<emインビボ></em>開裂により同時翻訳アクティベーションの使用(CoTrAC)
機能の研究、相互作用、および翻訳後修飾のため出芽酵母タンパク質の抽出と精製
シロイヌナズナタンパク質-DNA相互作用の同定のための免疫沈降アッセイクロマチン<em>インビボ</em
ChIP-エキソ方法:近く塩基対の精度でタンパク質 - DNA相互作用を同定します
3D磁力アクチュエータと多機能蛍光イメージングを組み合わせて核メカノバイオロジーを研究
Read Article