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Chemistry

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Hot Biologisk katalyse
 
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Hot Biologisk katalyse: Isothermal Titrering kalorimetri at karakterisere enzymatiske reaktioner

Article DOI: 10.3791/51487-v 13:00 min April 4th, 2014
April 4th, 2014

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Summary

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Click here for the English version.

Isotermisk titrering kalorimetri foranstaltninger varmestrøm frigivet eller absorberet i kemiske reaktioner. Denne metode kan anvendes til at kvantificere enzym-katalyse. I dette papir, protokol for instrumental opsætning, eksperiment kører, og dataanalyse beskrives generelt, og anvendes til karakterisering af enzymatisk urea hydrolyse ved sværdbønne urease.

Tags

Kemi Isotermisk titrering kalorimetri enzymatisk katalyse kinetik termodynamik entalpi Michaelis konstant katalytisk hastighedskonstant urease
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