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Hot Catalisi biologica: Calorimetria isotermica di titolazione per caratterizzare reazioni enzimatiche

Article DOI: 10.3791/51487 • 13:00 min
April 4th, 2014 •
1, 1, 1
1Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna

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April 4th, 2014

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Isotermico misure titolazione calorimetria flusso di calore rilasciato o assorbito nelle reazioni chimiche. Questo metodo può essere utilizzato per quantificare enzima-catalisi. In questo documento, il protocollo per la configurazione strumentale, esperimento esecuzione e analisi dei dati è generalmente descritto, e applicato alla caratterizzazione di idrolisi enzimatica dell'urea da jack bean ureasi.

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