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完全性脊髓损伤和脑解剖协议后续Wholemount<em>原位</em>杂交苗种海七鳃鳗
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Neuroscience
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JoVE Journal Neuroscience
Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey
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完全性脊髓损伤和脑解剖协议后续Wholemount<em>原位</em>杂交苗种海七鳃鳗

DOI:
10.3791/51494-v

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08:11 min

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October 14, 2014

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, , ,
1Centre for Neuroregeneration, School of Biomedical Sciences,University of Edinburgh, 2Shriners Hospitals Pediatric Research Center (Center for Neural Repair and Rehabilitation),Temple University School of Medicine, 3Department of Neurology,Temple University School of Medicine

Chapters

  • 00:05Title
  • 01:22Preparing the Larval Sea Lamprey
  • 02:17Complete Spinal Cord Transection
  • 04:30Brain Dissection
  • 06:55Results: Expression of neogenin After Spinal Cord Transection
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

七鳃鳗完整的脊髓损伤后恢复运动。然而，一些脊髓投射神经元是很好的再生和其他人都没有。本文说明了技术的外壳海七鳃鳗幼体（最近转化成人），产生完全性脊髓横断和原位杂交准备wholemount大脑和脊髓的。

Tags

Spinal Cord InjuryRegenerationWholemount In Situ HybridizationLarval Sea LampreyBrainstem NeuronsPropriospinal AxonsAxon RegenerationNeuron-intrinsic MechanismsGene ExpressionComplete TransectionHousingAnesthesiaDissection Protocol

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