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Neuroscience
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完全性脊髓损伤和脑解剖协议后续Wholemount<em>原位</em>杂交苗种海七鳃鳗
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount
In Situ
Hybridization in Larval Sea Lamprey
Please note that all translations are automatically generated.
Click here for the English version.
完全性脊髓损伤和脑解剖协议后续Wholemount<em>原位</em>杂交苗种海七鳃鳗
DOI:
10.3791/51494-v
•
08:11 min
•
October 14, 2014
•
Antón Barreiro-Iglesias
,
Guixin Zhang
,
Michael E. Selzer
3
,
Michael I. Shifman
1
Centre for Neuroregeneration, School of Biomedical Sciences
,
University of Edinburgh
,
2
Shriners Hospitals Pediatric Research Center (Center for Neural Repair and Rehabilitation)
,
Temple University School of Medicine
,
3
Department of Neurology
,
Temple University School of Medicine
Chapters
00:05
Title
01:22
Preparing the Larval Sea Lamprey
02:17
Complete Spinal Cord Transection
04:30
Brain Dissection
06:55
Results: Expression of
neogenin
After Spinal Cord Transection
07:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
七鳃鳗完整的脊髓损伤后恢复运动。然而,一些脊髓投射神经元是很好的再生和其他人都没有。本文说明了技术的外壳海七鳃鳗幼体(最近转化成人),产生完全性脊髓横断和
原位
杂交准备wholemount大脑和脊髓的。
Tags
Spinal Cord Injury
Regeneration
Wholemount In Situ Hybridization
Larval Sea Lamprey
Brainstem Neurons
Propriospinal Axons
Axon Regeneration
Neuron-intrinsic Mechanisms
Gene Expression
Complete Transection
Housing
Anesthesia
Dissection Protocol
Article
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