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Journal
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Bioengineering
/
生物膜的微阵列用刮墨刀型组合方式形成
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
Please note that all translations are automatically generated.
Click here for the English version.
生物膜的微阵列用刮墨刀型组合方式形成
DOI:
10.3791/51501-v
•
07:56 min
•
May 08, 2014
•
Nathan J. Wittenberg
,
Timothy W. Johnson
,
Luke R. Jordan
,
Xiaohua Xu
,
Arthur E. Warrington
,
Moses Rodriguez
4
,
Sang-Hyun Oh
2
1
Department of Electrical and Computer Engineering
,
University of Minnesota
,
2
Department of Biomedical Engineering
,
University of Minnesota
,
3
Department of Neurology
,
Mayo Clinic College of Medicine
,
4
Department of Immunology
,
Mayo Clinic College of Medicine
Chapters
00:05
Title
01:27
Preparation of Vesicles
02:30
Formation of Spherical Supported Lipid Bilayers (SSLB)
03:43
Assembling the SSLB Array
04:32
Cholera Toxin Binding Assay
05:31
Typical Microwell Intensities and Array Applications
07:12
Conclusion
Summary
Automatic Translation
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Automatic Translation
支持的脂质双层和天然膜颗粒是方便的系统,可以近似细胞膜的性质和在各种分析战略中。这里,我们证明了制备微阵列支持的脂质双层包被的SiO
2
的有孔玻璃珠,磷脂囊泡或天然膜颗粒组成的方法。
Tags
Biomembrane Microarray
Supported Lipid Bilayers
Spherical Supported Lipid Bilayers
Squeegee-based Assembly
Microfabricated Microwells
Cholera Toxin
GM1 Ganglioside
Lipid Vesicles
Cellular Biomembranes
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