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Vaccinia Reporter Vira til kvantificering Viral Funktion på alle stadier af genekspression
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Immunology and Infection
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JoVE Journal Immunology and Infection
Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Vaccinia Reporter Vira til kvantificering Viral Funktion på alle stadier af genekspression

DOI:
10.3791/51522-v

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10:48 min

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May 15, 2014

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, , ,
1Department of Microbiology,Boston University School of Medicine

Chapters

  • 00:05Title
  • 01:29Multi-stage Reporter Viruses
  • 02:27Plate Cells
  • 05:08Fixing Cells
  • 06:13Quantify Virus Growth
  • 07:29Alternative Protocol: Kinetic Plate Assays
  • 08:29Effect of Poxvirus Inhibitors on TrpV Expression
  • 10:10Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver brugen af ​​en fluorescerende reporter vacciniavirus, der muliggør real-time måling af virusinfektivitet og genekspression igennem etapen-specifikt udtryk for spektralt adskilte reporter fluorophores. Vi detalje en plade-baserede metode til præcist at identificere det stadium, hvor virus replikation påvirkes som reaktion på små molekyle hæmning.

Tags

PoxvirusVaccinia VirusReporter VirusGene ExpressionFluorescent ProteinViral ReplicationHigh-throughputAntiviral DiscoveryVirus-host InteractionEvolutionary Biology

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