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Vaccinia Reporter Vira til kvantificering Viral Funktion på alle stadier af genekspression
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
DOI:

10:48 min

May 15, 2014

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Chapters

  • 00:05Title
  • 01:29Multi-stage Reporter Viruses
  • 02:27Plate Cells
  • 05:08Fixing Cells
  • 06:13Quantify Virus Growth
  • 07:29Alternative Protocol: Kinetic Plate Assays
  • 08:29Effect of Poxvirus Inhibitors on TrpV Expression
  • 10:10Conclusion

Summary

Automatic Translation

Vi beskriver brugen af ​​en fluorescerende reporter vacciniavirus, der muliggør real-time måling af virusinfektivitet og genekspression igennem etapen-specifikt udtryk for spektralt adskilte reporter fluorophores. Vi detalje en plade-baserede metode til præcist at identificere det stadium, hvor virus replikation påvirkes som reaktion på små molekyle hæmning.

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