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在离体血液灌注大鼠肺备量化单微血管通透性
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JoVE Journal Biology
Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation
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在离体血液灌注大鼠肺备量化单微血管通透性

DOI:
10.3791/51552-v

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07:22 min

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June 30, 2014

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,
1Department of Physiology,The University of Tennessee Health Science Center, 2Department of Orthopedic Surgery and Biomedical Engineering,The University of Tennessee Health Science Center

Chapters

  • 00:05Title
  • 01:07Isolating the Rat Lung With Attached Heart
  • 02:25Blood Perfusion of the Isolated Rat Lung
  • 03:40Microvascular Infusion of the Lung
  • 06:05Vascular Permeability after Bacterial Lipopolysaccharide Treatment
  • 06:58Conclusion

Summary

Automatic Translation

Automatic Translation

孤立的血液灌注肺的准备，使可视化微血管网络上的肺表面是可行的。在这里，我们描述的方法用实时荧光成像量化单一微​​血管隔离肺的通透性。

Tags

Single Microvessel PermeabilityIsolated Blood-perfused Rat LungReal-time ImagingPulmonary MicrovesselsPermeability ChangesFluorophore InfusionBacterial LipopolysaccharideVenular And Capillary Microvessel SegmentsLung PermeabilityLung Microvascular Permeability

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