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Kvantificering Single mikrokardannelse Permeability i Isoleret Blood-perfunderet rottelunge Forberedelse
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JoVE Journal Biology
Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation

Kvantificering Single mikrokardannelse Permeability i Isoleret Blood-perfunderet rottelunge Forberedelse

DOI:
10.3791/51552-v

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07:22 min

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June 30, 2014

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,
1Department of Physiology,The University of Tennessee Health Science Center, 2Department of Orthopedic Surgery and Biomedical Engineering,The University of Tennessee Health Science Center

Chapters

  • 00:05Title
  • 01:07Isolating the Rat Lung With Attached Heart
  • 02:25Blood Perfusion of the Isolated Rat Lung
  • 03:40Microvascular Infusion of the Lung
  • 06:05Vascular Permeability after Bacterial Lipopolysaccharide Treatment
  • 06:58Conclusion

Summary

Automatic Translation

Automatic Translation

Den isolerede lunge forberedelse blod perfunderet gør det muligt at visualisere mikrokar net på lungeoverfladen. Her beskriver vi en metode til at kvantificere permeabilitet af enlige mikrokar i isolerede lunger ved hjælp af real tid fluorescens billeddannelse.

Tags

Single Microvessel PermeabilityIsolated Blood-perfused Rat LungReal-time ImagingPulmonary MicrovesselsPermeability ChangesFluorophore InfusionBacterial LipopolysaccharideVenular And Capillary Microvessel SegmentsLung PermeabilityLung Microvascular Permeability

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