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Chapters
- 00:05Title
- 01:10Preparation of Electro-stimulator Apparatus
- 02:01Forceps Preparation
- 03:40Microcapillary Tube Preparation
- 04:35Spider Immobilization
- 06:19Venom Collection
- 08:10Venom Gland Dissection
- 09:36Results: Venom Characterization
- 10:02Conclusion
This article provides a protocol for the extraction of venom from spiders using electrical stimulation in order to 1) conduct proteomic characterization, 2) stimulate venom gland gene expression, and 3) perform functional studies of venoms. This is followed by a description of venom gland microdissections for gene expression studies.
