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Flat Mount Imaging af Mouse Hud og sin ansøgning til Analyse af hårsækken Patterning og sensoriske Axon Morfologi
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1Department of Molecular Biology and Genetics,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 2Department of Neuroscience,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 3Department of Ophthalmology,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine
Chapters
- 00:06Title
- 02:01Dorsal Skin for Immunostaining and Melanin Imaging
- 05:40Foot Skin for the Analysis of Hair Follicle Patterning
- 06:42Foot Skin for Visualizing Sebaceous Glands
- 07:23Tail Skin Preparation for the Analysis of Melanin Distribution, Hair Follicle Orientation, and Sebaceous Gland Visualization
- 08:54Imaging Hair Follicle Orientation
- 09:52Imaging and Tracing Axon Arbors
- 10:55Results: Geometry of Various Skin Structures Are Easily Visualized in Skin Flatmounts
- 12:51Conclusion
Pattedyrs hud indeholder en bred vifte af strukturer - såsom hårsækkene og nerveender - der udviser karakteristiske mønstre af rumlig organisation. Analyse af huden som en flad montering drager fordel af 2-dimensional geometri dette væv til at producere fuld tykkelse billeder af hud strukturer med høj opløsning.
