Journal
/
Biology
/
培养<em>秀丽隐杆线虫</em >在无菌液体介质与创造转基因蠕虫通过微粒轰击
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Culturing Caenorhabditis elegans in Axenic Liquid Media and Creation of Transgenic Worms by Microparticle Bombardment
Please note that all translations are automatically generated. Click here for the English version.

培养<em>秀丽隐杆线虫</em >在无菌液体介质与创造转基因蠕虫通过微粒轰击

DOI:
10.3791/51796-v

•

08:26 min

•

August 02, 2014

•

, , ,
1Department of Animal and Avian Sciences,University of Maryland, 2Department of Cell Biology and Molecular Genetics,University of Maryland

Chapters

  • 00:05Title
  • 01:26Preparation of Modified C. elegans Habituation and Reproduction Medium (mCeHR)
  • 02:50Preparing C. elegans for mCeHR Culture
  • 05:39Synchronizing and Storing Worms in mCeHR Culture
  • 06:55Results: Nutrient Studies and Trangene Delivery in mCeHR
  • 07:51Conclusion

Summary

Automatic Translation

Automatic Translation

线虫通常生长在固体琼脂平板或液体培养基接种大肠杆菌大肠杆菌 。为了防止细菌副产品混杂毒理学和营养研究，我们利用的无菌液体介质，CeHR，成长和同步大量的蠕虫病毒对各种下游应用。

Tags

Caenorhabditis ElegansAxenic Liquid MediaMCeHR MediaMicroparticle BombardmentTransgenic WormsNutrient RequirementsHeme ConcentrationFluorescent SensorsGrowth And DevelopmentToxicological Effects

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

测量线虫固体介质的寿命

生成的基因 C。线虫通过基因枪转化

分离和<em在体外激活线虫精子

时间推移显微镜早期胚胎发育线虫

基本的<em>线虫</em>方法：同步和观察

前列腺素的提取与分析<em>线虫</em

人鼻粘膜上皮细胞在气液界面培养

可筛选的<em>在体内</em>测定线粒体调节剂使用转基因生物发光<em>秀丽隐杆线虫</em

栽培<em>秀丽隐杆线虫</em>在三个维度实验室

线虫的耗氧率测定

Read Article