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En ny metode til lokalisering Reporter fluorescerende kugler nærheden cellekulturen overflade for trækkraft Microscopy
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy
DOI:

13:30 min

September 16, 2014

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Chapters

  • 00:05Title
  • 01:42Functionalizing the Top Glass Cover Slips
  • 03:18Preparing Polyacrylamide Gel Directly on Glass Bottom Petri Dishes
  • 06:31Functionalizing Polyacrylamide Gel with Fibronectin
  • 07:47Traction Force Experiments
  • 10:22Results: Localizing Fluorescent Microspheres within Polyacrylamide Gels for Traction Force Microscopy
  • 12:51Conclusion

Summary

Automatic Translation

Traditionelle teknikker til fremstilling af polyacrylamid (PA), geler, der indeholder fluorescerende prober indebærer sandwiching en gel mellem en Tilhænger overflade og en glasplade. Her viser vi, at overtrække denne objektglas med poly-D-lysin (PDL) og fluorescerende prober lokaliserer prober inden for 1,6 um fra geloverfladen.

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