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多光子细胞内钠成像结合谷氨酸海马CA1锥体神经元的UV-介导的焦点解笼锁
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
Please note that all translations are automatically generated.
Click here for the English version.
多光子细胞内钠成像结合谷氨酸海马CA1锥体神经元的UV-介导的焦点解笼锁
DOI:
10.3791/52038-v
•
10:29 min
•
October 08, 2014
•
Christian Kleinhans*
1
,
Karl W. Kafitz*
1
,
Christine R. Rose
1
Institute of Neurobiology
,
Heinrich Heine University Düsseldorf
Chapters
00:05
Title
01:56
Dissection of Tissue
03:05
Preparation of Hardware
05:03
Whole-cell Patch-clamping
06:11
Multi-photon Imaging and Stimulation
08:05
Results: Sodium Signals and Synaptic Currents Induced by Flash Photolysis of Caged Glutamate
09:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了焦紫外线引起的光激活的神经活性化合物与全细胞膜片钳和在枝晶和海马神经元的棘在小鼠脑的急性组织切片的细胞内钠瞬变多光子成像的组合。
Tags
Multi-photon Imaging
Sodium Imaging
UV-mediated Uncaging
Glutamate Uncaging
CA1 Pyramidal Neurons
Whole-cell Patch-clamp
SBFI
MNI-glutamate
Ionotropic Glutamate Receptors
Dendritic Spines
Hippocampus
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