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多光子细胞内钠成像结合谷氨酸海马CA1锥体神经元的UV-介导的焦点解笼锁
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
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多光子细胞内钠成像结合谷氨酸海马CA1锥体神经元的UV-介导的焦点解笼锁

DOI:
10.3791/52038-v

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10:29 min

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October 08, 2014

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, ,
1Institute of Neurobiology,Heinrich Heine University Düsseldorf

Chapters

  • 00:05Title
  • 01:56Dissection of Tissue
  • 03:05Preparation of Hardware
  • 05:03Whole-cell Patch-clamping
  • 06:11Multi-photon Imaging and Stimulation
  • 08:05Results: Sodium Signals and Synaptic Currents Induced by Flash Photolysis of Caged Glutamate
  • 09:43Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了焦紫外线引起的光激活的神经活性化合物与全细胞膜片钳和在枝晶和海马神经元的棘在小鼠脑的急性组织切片的细胞​​内钠瞬变多光子成像的组合。

Tags

Keywords: Multi-photon ImagingSodium ImagingUV-mediated UncagingGlutamate UncagingCA1 Pyramidal NeuronsWhole-cell Patch-clampSBFIMNI-glutamateIonotropic Glutamate ReceptorsDendritic SpinesHippocampus

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