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支持的脂双层云母制备的高分辨率光学显微成像
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Bioengineering
Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
Please note that all translations are automatically generated.
Click here for the English version.
支持的脂双层云母制备的高分辨率光学显微成像
DOI:
10.3791/52054-v
•
07:48 min
•
June 07, 2014
•
Artur Matysik
,
Rachel S. Kraut
1
School of Biological Sciences
,
Nanyang Technological University
Chapters
00:01
Title
00:47
Mica and Slides Preparation
02:26
Mica Assembly and Chamber Mounting
05:10
Supported Lipid Bilayer (SLB) Formation
05:45
Results: Diffusion Analysis of Glass and Mica Supported DOPC Bilayers
07:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了制备云母支持的脂质双层膜为高分辨率显微镜的方法。云母是在原子尺度透明且平坦,但很少采用,因为处理困难成像;我们的制剂导致的云母薄片甚至沉积,并且降低了在双分子层制备中使用的材料。
Tags
Supported Lipid Bilayers
Mica
High-resolution Optical Microscopy
SLB Preparation
SLB Imaging
Lipid Vesicle Deposition
Custom-made Chamber
AFM
Single-molecule Fluorescence Microscopy
FCS
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