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JoVE Journal
Bioengineering

This content is Open Access.

High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network
 

High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network

Article DOI: 10.3791/56859
January 29th, 2018

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Summary January 29th, 2018

Here, we present a protocol to image human pancreas sections in three dimensions (3D) using optimized passive clearing methods. This manuscript demonstrates these procedures for passive optical clearing followed by multiple immunofluorescence staining to identify key elements of the autonomic and sensory neural networks innervating human islets.

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