High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network

Elizabeth Butterworth; Wesley Dickerson; Vindhya Vijay; Kristina Weitzel; Julia Cooper; Eric W. Atkinson; Jason E. Coleman; Kevin J. Otto; Martha Campbell-Thompson

doi:10.3791/56859

Journal

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Bioengineering

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High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network

10,982 Views

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09:54 min

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January 29, 2018

DOI:

10.3791/56859-v

DOI:

10.3791/56859-v

•

09:54 min

January 29, 2018

•

10967 Views

Elizabeth Butterworth¹, Wesley Dickerson², Vindhya Vijay³, Kristina Weitzel¹, Julia Cooper¹, Eric W. Atkinson⁴, Jason E. Coleman⁵, Kevin J. Otto⁴, Martha Campbell-Thompson¹

¹Department of Pathology, Immunology and Experimental Medicine,University of Florida ²Heller School for Social Policy and Management,Brandeis University ³Department of Medicine, College of Medicine,University of Florida ⁴Department of Biomedical Engineering, College of Engineering,University of Florida ⁵Department of Pediatrics, College of Medicine,University of Florida

Summary

Here, we present a protocol to image human pancreas sections in three dimensions (3D) using optimized passive clearing methods. This manuscript demonstrates these procedures for passive optical clearing followed by multiple immunofluorescence staining to identify key elements of the autonomic and sensory neural networks innervating human islets.