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High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network
High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network

High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network

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09:54 min

January 29, 2018

DOI:

09:54 min
January 29, 2018

10967 Views
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Summary

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Here, we present a protocol to image human pancreas sections in three dimensions (3D) using optimized passive clearing methods. This manuscript demonstrates these procedures for passive optical clearing followed by multiple immunofluorescence staining to identify key elements of the autonomic and sensory neural networks innervating human islets.

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