Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Bioengineering

This content is Open Access.

JoVE Journal Bioengineering
High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network
 

High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network

Article doi: 10.3791/56859
January 29th, 2018 •
1, 2, 3, 1, 1, 4, 5, 4, 1
1Department of Pathology, Immunology and Experimental Medicine, University of Florida, 2Heller School for Social Policy and Management, Brandeis University, 3Department of Medicine, College of Medicine, University of Florida, 4Department of Biomedical Engineering, College of Engineering, University of Florida, 5Department of Pediatrics, College of Medicine, University of Florida

Summary
Automatic Translation
January 29th, 2018

Here, we present a protocol to image human pancreas sections in three dimensions (3D) using optimized passive clearing methods. This manuscript demonstrates these procedures for passive optical clearing followed by multiple immunofluorescence staining to identify key elements of the autonomic and sensory neural networks innervating human islets.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter