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评估白细胞内皮的相互作用下水流条件的<em>离体</em>自动注入微流室法
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an
Ex Vivo
Autoperfused Microflow Chamber Assay
Please note that all translations are automatically generated.
Click here for the English version.
评估白细胞内皮的相互作用下水流条件的<em>离体</em>自动注入微流室法
DOI:
10.3791/52130-v
•
09:01 min
•
December 30, 2014
•
Lama Mulki*
1,2
,
J. Harry Sweigard*
1
,
Kip M. Connor
1
Angiogenesis Laboratory, Department of Ophthalmology
,
Harvard Medical School, Massachusetts Eye & Ear Infirmary
,
2
St. Elizabeth’s Medical Center
Chapters
00:05
Title
01:19
Coating the Chamber
02:35
Filling the Chamber
03:41
Connecting the Chamber to the Mouse
06:04
Recording Rolling Velocity
07:32
Results: Representative Time Course of Leukocytes Rolling Through the Flow Chamber
08:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一个协议,允许调查人员通过连接涂上内皮源性黏附分子鼠标循环系统室,以评估白细胞招聘动态
体外
。此方法提供了显著优势,因为它允许相对生物学条件下白细胞的评估。
Tags
Leukocyte-endothelial Interactions
Ex Vivo Autoperfused Microflow Chamber Assay
Inflammation
Peripheral Blood Leukocytes (PBLs)
Adhesion Molecules
Intravital Microscopy
Flow Conditions
Leukocyte Rolling Velocity
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