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Chapters
- 00:05Title
- 01:21Preparation of Organotypic Slices
- 02:20Preparation of DNA-coated Micro & Nano-projectiles
- 03:38Biolistic Transfection on Organotypic Slices
- 04:29Fixing and Visualization of Brain Slices
- 05:24Results: Biolistic Delivery of Fluorescent Protein Encoded DNA into Organotypic Slices
- 06:12Conclusion
Recent improvements in organotypic brain slice preparations have permitted their exploitation for biotechnological applications. Organotypic slices maintain local structural characteristics of in vivo biology, including functional synaptic connections. Here we present a regioselective biolistic delivery method to label and genetically manipulate these slices.
