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Subclonación Plus Inserción (SPI) - Un nuevo método recombinería para la rápida construcción de la orientación de genes Vectores
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Subcloning Plus Insertion (SPI) – A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
DOI:

09:02 min

January 08, 2015

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Chapters

  • 00:05Title
  • 01:04Multiplex Recombineering Oligos
  • 02:00BAC Clone Transformation
  • 03:56Insertion Cassettes and Subcloning Plasmids
  • 05:03Subcloning Plus Insertion
  • 07:07Analysis of Recombinants
  • 07:48Results: Subcloning Plus Insertion (SPI)
  • 08:39Conclusion

Summary

Automatic Translation

Gene targeting methodologies can be used to generate transgenic mice with knockout, knock-in and tagged alleles. Here, we describe an improved method of recombineering in E. coli, that we term ‘subcloning plus insertion’, which can be used to generate custom gene targeting vectors rapidly.

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