Journal
/
Developmental Biology
/
Efficient Derivation of Retinal Pigment Epithelium Cells from Stem Cells
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Efficient Derivation of Retinal Pigment Epithelium Cells from Stem Cells

Efficient Derivation of Retinal Pigment Epithelium Cells from Stem Cells

DOI:
10.3791/52214-v

•

07:07 min

•

March 08, 2015

•

, , ,
1Department of Cell and Molecular Biology,The Scripps Research Institute, 2Lowy Medical Research Institute

Chapters

  • 00:05Title
  • 01:47Directed Differentiation of Stem Cell-derived Retinal Pigment Epithelium (RPE)
  • 02:51Isolating Pigmentation Islets
  • 03:59Passaging Stem Cell-derived RPE
  • 05:42Results: Representative iPS-RPE Culture and Expansion
  • 06:19Conclusion

Summary

Automatic Translation

Automatic Translation

Stem cell-derived retinal pigment epithelium (RPE) cells may be used for multiple applications including cell-based therapies for retinal degeneration, disease modeling, and drug studies. Here we present a simple protocol for reproducibly deriving RPE from stem cells.

Tags

Keywords: Retinal Pigment EpitheliumRPEAge-related Macular DegenerationAMDStem Cell DifferentiationIPS-RPENicotinamideActivin AIDE-1Cell-based Therapy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52214?article_type=v

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells
check_url/52214?article_type=v

Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
check_url/52214?article_type=v

Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
check_url/52214?article_type=v

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin
check_url/52214?article_type=v

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
check_url/52214?article_type=v

Single-cell Photoconversion in Living Intact Zebrafish
check_url/52214?article_type=v

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
check_url/52214?article_type=v

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells
check_url/52214?article_type=v

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
check_url/52214?article_type=v

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Read Article