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Genoom-brede Eiwit-eiwit Interactie Screening door Eiwit-fragment Complementatie Assay (PCA) in levende cellen
JoVE Journal
Biology
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JoVE Journal Biology
Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Genoom-brede Eiwit-eiwit Interactie Screening door Eiwit-fragment Complementatie Assay (PCA) in levende cellen

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08:38 min

March 03, 2015

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08:38 min
March 03, 2015

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Summary

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Proteins interact with each other and these interactions determine in a large part their functions. Protein interaction partners can be identified at high-throughput in vivo using a yeast fitness assay based on the dihydrofolate reductase protein-fragment complementation assay (DHFR-PCA).

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