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跨膜蛋白的重组,电压门控离子通道,KvAP,进入巨人单层囊的显微镜和膜片钳研究
JoVE Journal
Biology
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JoVE Journal Biology
Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies
DOI:

11:42 min

January 22, 2015

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Chapters

  • 00:05Title
  • 01:44Giant Unilamellar Vesicle Growth by Electroformation
  • 04:29Giant Unilamellar Vesicle Growth by Gel-assisted Swelling
  • 06:07Harvesting and Transferring GUVs
  • 07:33Patch-clamping GUVs
  • 09:14Results: Microscopy and Patch Clamp Studies of KvAP GUVs
  • 11:03Conclusion

Summary

Automatic Translation

跨膜蛋白,KvAP,变成巨大的单层囊泡(GUVs)的重组被证明两脱水补液方法 - electroformation和凝胶辅助肿胀。在两种方法中,将含有蛋白的小单层囊泡融合在一起,以形成可以随后通过荧光显微镜和膜片钳电生理学来研究GUVs。

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