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1Institut Curie, Centre de Recherche, CNRS, UMR 168, PhysicoChimie Curie,Université Pierre et Marie Curie, 2Kavli Institute for Brain and Mind,University of California, San Diego, 3Molecular Physiology and Biophysics Section, National Institute for Neurological Disorders and Stroke,National Institute of Health
Chapters
- 00:05Title
- 01:44Giant Unilamellar Vesicle Growth by Electroformation
- 04:29Giant Unilamellar Vesicle Growth by Gel-assisted Swelling
- 06:07Harvesting and Transferring GUVs
- 07:33Patch-clamping GUVs
- 09:14Results: Microscopy and Patch Clamp Studies of KvAP GUVs
- 11:03Conclusion
Den beredning av transmembranproteinet, KvAP, till gigantiska enlamellvesiklar (GUVs) demonstreras för två uttorkning-rehydrering metoder - electroformation och gel-assisterad svullnad. I båda metoderna, är små unilamellära vesiklar innehållande proteinet smält samman för att bilda GUVs som sedan kan studeras genom fluorescensmikroskopi och patch-clamp-elektrofysiologi.
Tags
Transmembrane ProteinVoltage-gated Ion ChannelKvAPGiant Unilamellar Vesicles (GUVs)Biomimetic SystemMembrane Associated PhenomenaDehydration-rehydrationElectroformationGel-assisted SwellingSmall Unilamellar VesiclesRehydration BufferAC FieldAgarose GelFluorescence MicroscopyPatch-clampProtein Distribution
