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使用高通量的<em>在体外</em>微流控系统开发口服多物种生物膜

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Please note that all translations are automatically generated. Click here for the English version.

使用高通量的<em>在体外</em>微流控系统开发口服多物种生物膜

DOI:

10.3791/52467-v

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07:09 min

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December 01, 2014

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Derek S. Samarian, Nicholas S. Jakubovics, Ting L. Luo, Alexander H. Rickard

¹Department of Epidemiology, School of Public Health,The University of Michigan, ²Centre for Oral Health Research, School of Dental Sciences,Newcastle University

Chapters

00:05Title
02:06Preparation of Pooled Human Saliva as a Bacteria-positive Inoculum
02:51Growth and Live-dead Staining of Biofilms in a Microfluidic Chip
05:57Results: Live-dead Staining of Oral Cavity Biofilms Grown in Microfluidic Chips
06:43Conclusion

Summary

Automatic Translation

此方法本文的目的是描述了使用微流体系统的为包含通常在人类龈上菌斑鉴定物种多品种生物膜的发展。方法描述生物膜结构，生物膜的可行性，以及一种方法来收获生物膜文化依赖或培养独立分析突出显示。

Tags

High-throughput In Vitro Microfluidic System Multi-species Biofilms Oral Biofilms Human Saliva Supragingival Dental Plaque Confocal Laser Scanning Microscopy 3D Biofilm Reconstruction Biofilm Architecture Biofilm Viability Streptococcus Neisseria Veillonella Gemella Porphyromonas Biofilm Cell Harvesting DNA Extraction Data Analysis

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