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Journal
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Developmental Biology
/
斑马鱼角膜植研究集体细胞迁移和再上皮化在皮肤伤口愈合
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Developmental Biology
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JoVE Journal
Developmental Biology
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
Please note that all translations are automatically generated.
Click here for the English version.
斑马鱼角膜植研究集体细胞迁移和再上皮化在皮肤伤口愈合
DOI:
10.3791/52489-v
•
09:35 min
•
February 25, 2015
•
Jose L. Rapanan
,
Agnes S. Pascual
,
Chandana K. Uppalapati
,
Kimbal E. Cooper
,
Kathryn J. Leyva
,
Elizabeth E. Hull
1
Biomedical Sciences Program
,
Midwestern University
,
2
Department of Microbiology & Immunology
,
Midwestern University
Chapters
00:05
Title
01:55
Preparation of Anesthesia, Equipment, and Media
02:55
Establish Explant Cultures
04:40
Brightfield and Video Microscopy
05:20
Fixation for Immunofluorescence Microscopy
07:02
Results: Cell Migration in Zebrafish Keratocytes
08:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
斑马鱼角膜基质细胞的表外植迁移,并提供了一个
体外
模型集体细胞迁移的上皮伤口愈合的情况下机制的研究。这些协议的细节,以建立初级外植体培养在集体细胞迁移测定法使用的有效方式。
Tags
Zebrafish
Keratocyte
Explant
Collective Cell Migration
Reepithelialization
Cutaneous Wound Healing
Single Cell Migration
Actin-rich Lamellae
Actin Cable
Epithelial To Mesenchymal Transition
Gene Expression
Mutant
Transgenic
In Vitro Model
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