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Sebrafisk Keratocyte eksplantater å studere Collective Cell Migrasjon og reepithelialization i Kutan Wound Healing
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
DOI:

09:35 min

February 25, 2015

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Chapters

  • 00:05Title
  • 01:55Preparation of Anesthesia, Equipment, and Media
  • 02:55Establish Explant Cultures
  • 04:40Brightfield and Video Microscopy
  • 05:20Fixation for Immunofluorescence Microscopy
  • 07:02Results: Cell Migration in Zebrafish Keratocytes
  • 08:40Conclusion

Summary

Automatic Translation

Sebrafisk keratocytter migrere i celle ark fra eksplantater og gi en in vitro modell for studiet av mekanismene for kollektiv cellemigrasjon i sammenheng med epitel sårtilheling. Disse protokoller detalj en effektiv måte å etablere primære eksplantering kulturer for anvendelse i kollektive cellemigrerings analyser.

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