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Journal / Immunology and Infection / Udnytte den Antigen capsidet-Indbygning strategi…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Udnytte den Antigen capsidet-Indbygning strategi for udvikling af adenovirusserotype 5-vektoriserede Vaccine Approaches
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Udnytte den Antigen capsidet-Indbygning strategi for udvikling af adenovirusserotype 5-vektoriserede Vaccine Approaches

Article DOI: 10.3791/52655-v • 13:36 min • May 6th, 2015
May 6th, 2015 •
1, 1, 1, 1,2
1Department of Medicine, University of Alabama at Birmingham, 2Center for AIDS Research, University of Alabama at Birmingham

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Her præsenterer vi en protokol til at generere en proof-of-principle divalente adenovirus type 5 (Ad5) vektor Ad5 / H5-HVR1-Kwas-HVR5-His 6 ved at udnytte Antigen capsidet-Indbygning-strategi. Denne vektor blev påvist at udvise kvalitativ egnethed skal muligheden undslippe Ad5-positive sera in vitro, og antigeniciteten samt immunogenicitet til de inkorporerede antigener.

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Immunologi Antigen capsidet-Indbygning strategi transgen metode Adenovirus (Ad) vaccine capsidproteinerne dual ændring allerede eksisterende immunitet (PEI)
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