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Bioengineering
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基于纸张的设备进行隔离外囊泡与表征
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
Please note that all translations are automatically generated.
Click here for the English version.
基于纸张的设备进行隔离外囊泡与表征
DOI:
10.3791/52722-v
•
11:53 min
•
April 03, 2015
•
Chihchen Chen
,
Bo-Ren Lin
,
Min-Yen Hsu
,
Chao-Min Cheng
1
Institute of Nanoengineering and Microsystems
,
National Tsing Hua University
,
2
Taichung Veterans General Hospital
Chapters
00:05
Title
01:18
Fabrication of Paper Devices
04:04
Serum and Aqueous Humor Sample Collection and Processing
05:09
Isolation of Extracellular Vesicles
05:56
Downstream Assay Example 1: Scanning Electron Micoscopy
07:04
Downstream Assay Example 2: Paper-based ELISA
08:04
Downstream Assay Example 3: RNA Isolation
10:26
Results: Characterization of Captured Extracellular Vesicles
11:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议细节的方法分离细胞外囊泡(电动汽车),从细胞中释放的小的膜的颗粒,从少至10微升血清样品。这种方法绕过需要超速离心,需要测定时间仅几分钟,并且使电动车从有限体积的样品的分离。
Tags
Extracellular Vesicles
EVs
Paper-based Devices
Immunoaffinity Platform
EV Isolation
EV Characterization
Scanning Electron Microscopy
P-ELISA
Transcriptome Analysis
Intercellular Communication
Clinical Applications
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