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基于纸张的设备进行隔离外囊泡与表征
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
DOI:

11:53 min

April 03, 2015

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Chapters

  • 00:05Title
  • 01:18Fabrication of Paper Devices
  • 04:04Serum and Aqueous Humor Sample Collection and Processing
  • 05:09Isolation of Extracellular Vesicles
  • 05:56Downstream Assay Example 1: Scanning Electron Micoscopy
  • 07:04Downstream Assay Example 2: Paper-based ELISA
  • 08:04Downstream Assay Example 3: RNA Isolation
  • 10:26Results: Characterization of Captured Extracellular Vesicles
  • 11:24Conclusion

Summary

Automatic Translation

该协议细节的方法分离细胞外囊泡(电动汽车),从细胞中释放的小的膜的颗粒,从少至10微升血清样品。这种方法绕过需要超速离心,需要测定时间仅几分钟,并且使电动车从有限体积的样品的分离。

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