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Chick Heart Invasion Assay til Test af invasiv af kræftceller og aktiviteten af ​​Potentielt Anti-invasive forbindelser
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JoVE Journal Medicine
Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Chick Heart Invasion Assay til Test af invasiv af kræftceller og aktiviteten af ​​Potentielt Anti-invasive forbindelser

DOI:
10.3791/52792-v

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10:16 min

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June 06, 2015

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, , , , , ,
1Department of Radiation Oncology and Experimental Cancer Research,University of Ghent, 2Department of Sustainable Organic Chemistry and Technology,University of Ghent, 3Department of Chemistry,University of Delhi

Chapters

  • 00:05Title
  • 02:05Pre-cultured Heart Fragment (PHF) Preparation
  • 05:16Spheroidal Test Cell Aggregate Preparation, Confrontation and Histology
  • 08:07Results: Toxicity and Growth Assessment of the Confronting Culture
  • 09:17Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en protokol til at studere invasionen af ​​tumorceller i levende normale væv fragmenter i tre dimensioner. Denne organkultur teknik anvendes hovedsagelig til at teste potentielt anti-invasive lægemidler in vitro.

Tags

Chick Heart Invasion AssayCancer Cell InvasivenessAnti-invasive CompoundsOrgan Culture3D InteractionCancer InvasionStromal EnvironmentQuantitative Structure-activity Relation (QSAR)FlavonoidsChalconesAnti-metastatic DrugsMicrotubule Inhibitors

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