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Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model
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JoVE Journal Medicine
Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model

Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model

DOI:
10.3791/52861-v

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05:22 min

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October 09, 2015

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, , , , , ,
1Department of Biological Sciences,Hunter College of The City University of New York, 2Departments of Biology and Biochemistry,The Graduate Center of The City University of New York, 3Department of Pathology, Immunology, and Laboratory Medicine,University of Florida

Chapters

  • 00:05Title
  • 01:10Collection of Whole Blood for Circulating Tumor Cell Isolation
  • 02:08Lysis of RBC in the Buffy Coat
  • 03:19Propagation of Circulating Tumor Cells
  • 04:29Results: Isolation of Circulating Tumor Cells
  • 04:56Conclusion

Summary

Automatic Translation

Automatic Translation

Circulating tumor cells (CTCs) have been shown to play an important role in tumor metastasis. Here, a method for the isolation and propagation of CTCs from the whole blood of a syngeneic mouse tumor model of hepatocellular carcinoma (HCC) metastasis is described.

Tags

Keywords: Circulating Tumor CellsCTC IsolationCTC PropagationMouse Cancer ModelHepatocellular CarcinomaMetastasisNon-invasive TechniquePersonalized Therapy

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